Princeton Separations CS-901 CENTRI-SEP Spin Columns 100 Column Kit

Description

This is a
Princeton Separations CS-901 CENTRI-SEP Spin Columns 100 Column Kit Best by 9/24/2020
What you will get:
Princeton Separations CS-901 CENTRI-SEP Spin Columns 100 Column Kit Best by 9/24/2020
These are my items 105, 106, 107, 108 and 109.  5 were available at listing time.  MSRP is 6.42
More Information:
Centri-Sep spin columns are the most effective and reliable column for removal of dye terminators prior to sequencing.
Centri-Sep columns are used for the fast and efficient purification of large molecules (proteins, nucleic acids, complex carbohydrates, etc.), from small molecules (nucleotides, buffer salts, etc.)
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Sep unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.
The Centri-Sep gel will provide excellent recovery of DNA fragments with sizes greater than 16 base pairs or 25-mer, while removing > 98 % of salts, NTP's and other unwanted low- molecular- weight impurities.
The column gel is hydrated with either reagent-grade water or a suitable buffer or your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low- molecular- weight components, but also exchanged into the buffer of your choice.
Centri-Sep Columns can also be used for the following applications:
Removal of excess DyeDeoxy™ terminators
Removal of excess BigDye™ terminators
Removal of more than 98% of salts and unwanted low-molecular-weight impurities
Purification of fluorescent reaction mixtures
Removal of free and labeled dNTP's from DNA or RNA, as inon:
nick
translation
end-labeling reactions
polymerization reaction
Removal of traces of phenol or exchange of buffer salts
Purification and desalting of proteins
Removal of excess nucleotides and small primers in PCR reactions
Removal of radioactive precursors
Desalting and buffer exchange
These columns are far superior – easy to use, quick protocol, non-toxicity – than phenol/chloroform extraction or ethanol precipitation.
Advantages of Centri-Sep spin column:
Processed faster than ultrafiltration columns
Rapid and efficient separations
Buffer is not preselected
Convenient 20-100 µL sample size
Dry gel format
Room Temperature Storage
Microcentrifuge format
Benefits of Centri-Sep spin column:
Reliable and consistent
Time-saving and convenient
Enhanced stability
Extended shelf-life
No special equipment
Extremely versatile.
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